Redirection of the transcription factor SP1 to AT rich binding sites by a synthetic adaptor molecule

  • The ubiquitous transcription factor SP1 binds to a GC rich consensus sequence. Here we describe an adaptor molecule that mediates binding of SP1 to a non-cognate DNA site rich in AT. The adaptor is comprised of a Dervan-type hairpin polyamide with high affinity to an AT rich hexamer duplex. It also carries a 27mer DNA that contains the SP1 consensus sequence. The synthesis and purification of the polyamide-DNA conjugate is reported. Pulldown experiments and western blot analysis demonstrate adaptor mediated binding of SP1 to the hexamer duplex TTGTTA.

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Author:Mathias Bolz, Ute Scheffer, Elisabeth Kalden, Michael GöbelORCiDGND
Parent Title (English):Helvetica chimica acta
Place of publication:New York, NY
Document Type:Article
Date of Publication (online):2021/08/08
Date of first Publication:2021/08/08
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2021/12/20
Tag:DNA conjugate; DNA recognition; adaptor mediated recruitment; hairpin polyamides; protein capture
Issue:9, art. e2100095
Page Number:9
First Page:1
Last Page:9
Open access funding enabled and organized by Projekt DEAL.
Institutes:Biochemie, Chemie und Pharmazie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - Namensnennung 4.0