Antibody-mediated neutralization of authentic SARS-CoV-2 B.1.617 variants harboring L452R and T478K/E484Q

  • The capacity of convalescent and vaccine-elicited sera and monoclonal antibodies (mAb) to neutralize SARS-CoV-2 variants is currently of high relevance to assess the protection against infections. We performed a cell culture-based neutralization assay focusing on authentic SARS-CoV-2 variants B.1.617.1 (Kappa), B.1.617.2 (Delta), B.1.427/B.1.429 (Epsilon), all harboring the spike substitution L452R. We found that authentic SARS-CoV-2 variants harboring L452R had reduced susceptibility to convalescent and vaccine-elicited sera and mAbs. Compared to B.1, Kappa and Delta showed a reduced neutralization by convalescent sera by a factor of 8.00 and 5.33, respectively, which constitutes a 2-fold greater reduction when compared to Epsilon. BNT2b2 and mRNA1273 vaccine-elicited sera were less effective against Kappa, Delta, and Epsilon compared to B.1. No difference was observed between Kappa and Delta towards vaccine-elicited sera, whereas convalescent sera were 1.51-fold less effective against Delta, respectively. Both B.1.617 variants Kappa (+E484Q) and Delta (+T478K) were less susceptible to either casirivimab or imdevimab. In conclusion, in contrast to the parallel circulating Kappa variant, the neutralization efficiency of convalescent and vaccine-elicited sera against Delta was moderately reduced. Delta was resistant to imdevimab, which, however, might be circumvented by combination therapy with casirivimab together.

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Author:Alexander WilhelmORCiDGND, Tuna ToptanORCiDGND, Christiane Pallas, Timo WolfORCiDGND, Udo GötschGND, René GottschalkGND, Maria J. G. T. VehreschildORCiDGND, Sandra CiesekORCiDGND, Marek WideraORCiDGND
Parent Title (English):Viruses
Place of publication:Basel
Document Type:Article
Date of Publication (online):2021/08/26
Date of first Publication:2021/08/26
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2021/11/26
Tag:B.1.617.1; B.1.617.2; BNT2b2; Epsilon; Kappa; SARS-CoV-2; corona virus; delta; mRNA1273; monoclonal antibodies; vaccination
Issue:9, art. 1693
Page Number:8
First Page:1
Last Page:8
This study has been performed with the support of the Goethe-Corona-Fund of the Goethe University Frankfurt (MW) and the Federal Ministry of Education and Research (COVIDready; grant 02WRS1621C (MW).
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0