Versatile vibrational energy sensors for proteins

  • Vibrational energy transfer (VET) is emerging as key mechanism for protein functions, possibly playing an important role for energy dissipation, allosteric regulation, and enzyme catalysis. A deep understanding of VET is required to elucidate its role in such processes. Ultrafast VIS-pump/IR-probe spectroscopy can detect pathways of VET in proteins. However, the requirement of having a VET donor and a VET sensor installed simultaneously limits the possible target proteins and sites; to increase their number we compare six IR labels regarding their utility as VET sensors. We compare these labels in terms of their FTIR, and VET signature in VET donor-sensor dipeptides in different solvents. Furthermore, we incorporated four of these labels in PDZ3 to assess their capabilities in more complex systems. Our results show that different IR labels can be used interchangeably, allowing for free choice of the right label depending on the system under investigation and the methods available.
Author:Jan Gerrit LöfflerORCiDGND, Erhan DenizORCiD, Carolin FeidORCiD, Valentin G. Franz, Jens BredenbeckORCiD
Parent Title (German):Angewandte Chemie. International Edition
Place of publication:Weinheim
Document Type:Article
Date of Publication (online):2022/02/28
Date of first Publication:2022/02/28
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2023/09/15
Tag:Non-Canonical Amino Acids; Protein Dynamics; Protein Modifications; Time-Resolved Spectroscopy; Vibrational Energy Transfer
Issue:21, e202200648
Article Number:e202200648
Page Number:8
First Page:1
Last Page:8
We acknowledge funding by the Humboldt-Foundation (Sofja Kovalevskaja Award), as well as DFG funding (BR 3746/4-1, INST 161/722-1, GRK 1986 “CLiC”). Open Access funding enabled and organized by Projekt DEAL.
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 53 Physik / 530 Physik
5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International