Early immune response in foreign body reaction is implant/material specific

  • The design of novel biomaterials should directly influence the host-immune system and steer it towards high biocompatibility. To date, new implants/materials have been tested for biocompatibility in vitro in cell cultures and in vivo in animal models. The current methods do not reflect reality (cell cultures) or are very time-consuming and deliver results only after weeks (animal model). In this proof-of-concept study, the suitability of a Whole Blood Stimulation Assay (WBSA) in combination with a Protein Profiler Array (PPA), as a readily available and cost-effective screening tool, was investigated. Three different biomaterials based on poly(lactic-co-glycolic acid (PLGA), calcium sulphate/-carbonate (CS) and poly(methyl methacrylate) (PMMA) were exposed to native whole blood from three volunteers and subsequently screened with a PPA. Individual reproducible protein profiles could be detected for all three materials after 24 h of incubation. The most intense reaction resulted from the use of PLGA, followed by CS. If even marginal differences in implants can be reflected in protein profiles, the combination of WBSA and PPA could serve as an early biocompatibility screening tool in the development of novel biomaterials. This may also lead to a reduction in costs and the amount of animal testing required.

Download full text files

Export metadata

Author:Nicolas SöhlingGND, Muriel OndrekaGND, Kerstin Kontradowitz, Tobias Reichel, Ingo MarziORCiDGND, Dirk Markus HenrichORCiDGND
Parent Title (English):Materials
Place of publication:Basel
Document Type:Article
Date of Publication (online):2022/03/16
Date of first Publication:2022/03/16
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2023/12/06
Tag:PLGA; bone tissue engineering; foreign body reaction; scaffold
Issue:6, art. 2195
Article Number:2195
Page Number:18
First Page:1
Last Page:18
The project was funded by Heraeus Medical GmbH, Wehrheim, Germany (GCM-18579).
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 60 Technik / 600 Technik, Technologie
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International