Lena Maria Biehl, Fedja Farowski, Catharina Hilpert, Angela Nowag, Anne Kretzschmar, Nathalie Rihab Jazmati, Anastasia Tsakmaklis, Imke Wieters, Yascha Khodamoradi, Hilmar Wisplinghoff, Maria J. G. T. Vehreschild
- Background: The understanding of longitudinal changes in the urinary microbiota of healthy women and its relation to intestinal microbiota is limited.
Methods: From a cohort of 15 premenopausal women without known urogenital disease or current symptoms, we collected catheter urine (CU), vaginal and periurethral swabs, and fecal samples on four visits over six months. Additionally, ten participants provided CU and midstream urine (MU) to assess comparability. Urine was subjected to expanded culture. 16S rRNA gene sequencing was performed on all urine, fecal, and selected vaginal and periurethral samples. Sequence reads were processed (DADA2 pipeline) and analyzed using QIIME 2 and R.
Results: Relative abundances of urinary microbiota were variable over 6–18 months. The degree of intraindividual variability of urinary microbiota was higher than that found in fecal samples. Still, nearly half of the observed beta diversity of all urine samples could be attributed to differences between volunteers (R2 = 0.48, p = 0.001). After stratification by volunteer, time since last sexual intercourse was shown to be a factor significantly contributing to beta diversity (R2 = 0.14, p = 0.001). We observed a close relatedness of urogenital microbial habitats and a clear distinction from intestinal microbiota in the overall betadiversity analysis. Microbiota compositions derived from MU differed only slightly from CU compositions. Within this analysis of low-biomass samples, we identified contaminating sequences potentially stemming from sequencing reagents.
Conclusions: Results from our longitudinal cohort study confirmed the presence of a rather variable individual urinary microbiota in premenopausal women. These findings from catheter urine complement previous observations on temporal dynamics in voided urine. The higher intraindividual variability of urinary microbiota as compared to fecal microbiota will be a challenge for future studies investigating associations with urogenital diseases and aiming at identifying pathogenic microbiota signatures.
MetadatenAuthor: | Lena Maria BiehlORCiDGND, Fedja FarowskiORCiDGND, Catharina HilpertGND, Angela NowagORCiDGND, Anne KretzschmarGND, Nathalie Rihab JazmatiORCiDGND, Anastasia TsakmaklisORCiD, Imke WietersGND, Yascha KhodamoradiORCiD, Hilmar WisplinghoffGND, Maria J. G. T. VehreschildORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-627134 |
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DOI: | https://doi.org/10.1371/journal.pone.0262095 |
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ISSN: | 1932-6203 |
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Parent Title (English): | PLOS ONE |
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Publisher: | PLOS |
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Place of publication: | San Francisco, California, US |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2022/01/14 |
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Date of first Publication: | 2022/01/14 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2024/03/04 |
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Tag: | Antibiotics; Bacteria; Catheters; Gene sequencing; Gut bacteria; Microbiome; Ribosomal RNA; Urine |
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Volume: | 17 |
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Issue: | 1, art. e0262095 |
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Article Number: | e0262095 |
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Page Number: | 17 |
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First Page: | 1 |
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Last Page: | 17 |
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Note: | Data Availability: The dataset generated and analyzed in this study is available in the NCBI Sequence Read Archive under the BioProject accession number PRJNA649069. |
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Note: | Funding: This study was supported by a study grant from the German Research Foundation (DFG - https://www.dfg.de/), grant number BI 1899/1-1, awarded to Lena M. Biehl. |
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HeBIS-PPN: | 522185053 |
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Institutes: | Medizin |
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Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |
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