The induced membrane technique - the filling matters: evaluation of different forms of membrane filling with and without bone marrow mononuclear cells (BMC) in large femoral bone defects in rats

  • The Masquelet technique is used to treat large bone defects; it is a two-stage procedure based on an induced membrane. To improve the induced membrane process, demineralized bone matrix in granular (GDBM) and fibrous form (f-DBM) was tested with and without bone marrow mononuclear cells (BMC) as filling of the membrane against the gold standard filling with syngeneic cancellous bone (SCB). A total of 65 male Sprague–Dawley rats obtained a 5 mm femoral defect. These defects were treated with the induced membrane technique and filled with SCB, GDBM, or f-DBM, with or without BMC. After a healing period of eight weeks, the femurs were harvested and submitted for histological, radiological, and biomechanical analyses. The fracture load in the defect zone was lower compared to SCB in all groups. However, histological analysis showed comparable new bone formation, bone mineral density, and cartilage proportions and vascularization. The results suggest that f-DBM in combination with BMC and the induced membrane technique cannot reproduce the very good results of this material in large, non-membrane coated bone defects, nevertheless it supports the maturation of new bone tissue locally. It can be concluded that BMC should be applied in lower doses and inflammatory cells should be removed from the cell preparation before implantation.

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Author:René VerboketORCiDGND, Nicolas SöhlingGND, Myriam Widad HeilaniORCiDGND, Charlotte FremdlingGND, Alexander Schaible, Katrin SchröderORCiDGND, Jan C. BruneORCiDGND, Ingo MarziORCiDGND, Dirk HenrichORCiDGND
URN:urn:nbn:de:hebis:30:3-692805
DOI:https://doi.org/10.3390/biomedicines10030642
ISSN:2227-9059
Parent Title (English):Biomedicines
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Date of Publication (online):2022/03/10
Date of first Publication:2022/03/10
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2024/03/04
Tag:BMNC; Masquelet technique; bone regeneration; critical size defect; tissue engineering
Volume:10
Issue:3, art. 642
Article Number:642
Page Number:17
First Page:1
Last Page:17
Note:
This research was in part funded by the German Institute for Cell and Tissue Replacement (DIZG, gemeinnützige GmbH), 12555 Berlin, Germany.
Institutes:Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International