Aging of podospora anserina leads to alterations of OXPHOS and the induction of non-mitochondrial salvage pathways

  • The accumulation of functionally impaired mitochondria is a key event in aging. Previous works with the fungal aging model Podospora anserina demonstrated pronounced age-dependent changes of mitochondrial morphology and ultrastructure, as well as alterations of transcript and protein levels, including individual proteins of the oxidative phosphorylation (OXPHOS). The identified protein changes do not reflect the level of the whole protein complexes as they function in-vivo. In the present study, we investigated in detail the age-dependent changes of assembled mitochondrial protein complexes, using complexome profiling. We observed pronounced age-depen-dent alterations of the OXPHOS complexes, including the loss of mitochondrial respiratory supercomplexes (mtRSCs) and a reduction in the abundance of complex I and complex IV. Additionally, we identified a switch from the standard complex IV-dependent respiration to an alternative respiration during the aging of the P. anserina wild type. Interestingly, we identified proteasome components, as well as endoplasmic reticulum (ER) proteins, for which the recruitment to mitochondria appeared to be increased in the mitochondria of older cultures. Overall, our data demonstrate pronounced age-dependent alterations of the protein complexes involved in energy transduction and suggest the induction of different non-mitochondrial salvage pathways, to counteract the age-dependent mitochondrial impairments which occur during aging.

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Metadaten
Author:Verena WarnsmannORCiDGND, Jana Meisterknecht, Ilka WittigORCiD, Heinz D. OsiewaczORCiDGND
URN:urn:nbn:de:hebis:30:3-692939
DOI:https://doi.org/10.3390/cells10123319
ISSN:2073-4409
Parent Title (English):Cells
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Date of Publication (online):2021/11/26
Date of first Publication:2021/11/26
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2024/05/06
Tag:OXPHOS; Podospora anserina; aging; complexome profiling
Volume:10
Issue:12, art. 3319
Article Number:3319
Page Number:19
First Page:1
Last Page:19
Note:
This work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) Os75/17-2 to H.D.O. and Wi3728/1-1 to I.W.
Note:
Data Availability Statement
The complexome profiling data are available in ProteomeXchange (http://www.proteomexchange.org/) via the PRIDE [138] partner repository with the identifiers PXD029352.
Institutes:Biowissenschaften
Medizin
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International