Lifespan increase of podospora anserina by oleic acid is linked to alterations in energy metabolism, membrane trafficking and autophagy

  • The maintenance of cellular homeostasis over time is essential to avoid the degeneration of biological systems leading to aging and disease. Several interconnected pathways are active in this kind of quality control. One of them is autophagy, the vacuolar degradation of cellular components. The absence of the sorting nexin PaATG24 (SNX4 in other organisms) has been demonstrated to result in impairments in different types of autophagy and lead to a shortened lifespan. In addition, the growth rate and the size of vacuoles are strongly reduced. Here, we report how an oleic acid diet leads to longevity of the wild type and a PaAtg24 deletion mutant (ΔPaAtg24). The lifespan extension is linked to altered membrane trafficking, which abrogates the observed autophagy defects in ΔPaAtg24 by restoring vacuole size and the proper localization of SNARE protein PaSNC1. In addition, an oleic acid diet leads to an altered use of the mitochondrial respiratory chain: complex I and II are bypassed, leading to reduced reactive oxygen species (ROS) production. Overall, our study uncovers multiple effects of an oleic acid diet, which extends the lifespan of P. anserina and provides perspectives to explain the positive nutritional effects on human aging.

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Author:Lea SchürmannsORCiDGND, Andrea HamannORCiD, Heinz D. OsiewaczORCiDGND
URN:urn:nbn:de:hebis:30:3-692958
DOI:https://doi.org/10.3390/cells11030519
ISSN:2073-4409
Parent Title (English):Cells
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Date of Publication (online):2022/02/02
Date of first Publication:2022/02/02
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2024/05/06
Tag:ATG24; ER; Podospora anserina; aging; autophagy; membrane trafficking; mitochondria; peroxisomes
Volume:11
Issue:3, art. 519
Article Number:519
Page Number:23
First Page:1
Last Page:23
Note:
This research was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)—Project-ID 25913077–SFB1177 to H.D.O.
Institutes:Biowissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International