Phosphoenolpyruvatcarboxykinase aus Phycomyces blakesleeanus Bgff.
Phosphoenolpyruvate carboxykinase in Phycomyces blakesleeanus Bgff.
- Phosphoenolpyruvate carboxykinase (PEPCK) from Phycomyces blakesleeanus was partially purified by protamine sulfate precipitation, ammoniumsulfate precipitation, and diethylamino ethyl cellulose (DEAE) treatment. This preparation was employed for the characterization of the enzyme. The Km values for phosphoenolpyruvate (PEP) and ADP were determined as 1.6 and 0.42 mᴍ. The nucleotid specifity was demonstrated for ADP exclusively. The use of sulfuryl reagents showed the presence of thiol groups sensitive against p-hydroxymercuribenzoate but not effected by N-ethylmaleimide.
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| Author: | Gerhard SandmannORCiD, Wilhelm HilgenbergGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-720799 |
| DOI: | https://doi.org/10.1515/znc-1978-9-1011 |
| ISSN: | 0939-5075 |
| ISSN: | 1865-7125 |
| Parent Title (German): | Zeitschrift für Naturforschung, C |
| Publisher: | Verlag der Zeitschrift für Naturforschung |
| Place of publication: | Tübingen |
| Document Type: | Article |
| Language: | German |
| Date of Publication (online): | 2014/06/02 |
| Year of first Publication: | 1978 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2024/02/01 |
| Tag: | Km values; Nucleotid Specifity; Phosphoenolpyruvate Carboxykinase; Phycomyces blakesleeanus; Sulfuryl Reagents |
| Volume: | 33.1978 |
| Issue: | 9-10 |
| Page Number: | 4 |
| First Page: | 667 |
| Last Page: | 670 |
| Institutes: | Biowissenschaften |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0 |
