Reactions of Essential Sulfhydryl Residues of Dehydrogenases with Nicotinamide-(S-Methylmercury-Thioinosine) Dinucleotide
- Sulfhydryl Groups, Methylmercury Containing Inactivator, Coenzyme Analogue Nicotinamide-(S-methylmercury-thioinosine) dinucleotide was formed by reaction of nicotin amide-(6-thiopurine) dinucleotide with methylmercury chloride. The compound exhibits coenzyme properties in the test with LDH (Km=1.5 × 10-4 м , Vmax=12500) and LADH (Km=1.7 × 10-4 м, Vmax=27) and inactivates YADH and GAPDH. From incubations with LDH and LADH the mercury containing coenzyme could be regained by column chromatography. The compound seems to be qualified for the X-ray structure analysis of the coenzyme-enzyme complex for some dehyrogenases based on the proportion of the heavy metal.
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| Author: | Christoph Woenckhaus, Helmut Duchmann |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-721082 |
| DOI: | https://doi.org/10.1515/znc-1975-9-1002 |
| ISSN: | 0939-5075 |
| ISSN: | 1865-7125 |
| Parent Title (German): | Zeitschrift für Naturforschung, C |
| Publisher: | Verlag der Zeitschrift für Naturforschung |
| Place of publication: | Tübingen |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2014/06/02 |
| Year of first Publication: | 1975 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2024/01/30 |
| Tag: | Coenzyme Analogue; Methylmercury Containing Inactivator; Sulfhydryl Groups |
| Volume: | 30.1975 |
| Issue: | 9-10 |
| Page Number: | 3 |
| First Page: | 562 |
| Last Page: | 564 |
| Institutes: | Medizin |
| Biochemie, Chemie und Pharmazie / Biochemie und Chemie | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0 |
