Interaction between dehydrogenases and a new NAD⊕-isomer
- A new NAD⊕-isomer was prepared, in which the ᴅ-ribose of the adenosine moiety was sub stituted by the enantiomeric ʟ-ribose. As compared to nicotinamide-adenine-dinucleotide (NAD⊕) and NADH the coenzyme isomer (ᴅ,ʟ)-NAD⊕ and its dihydroform (ᴅ,ʟ)-NADH are far less tightly bound to lactate dehydrogenase and alcohol dehydrogenase from horse liver. In the presence of the second substrate (ᴅ,ʟ)-NAD⊕ and (ᴅ,ʟ)-NADH act as hydrogen acceptor and hydrogen donator, respectively, with lactate dehydrogenase and alcohol dehydrogenases from horse liver and yeast. Compared to NAD⊕ and NADH the Michaelis constants are always increased, the catalytic constants (V/Et) were found to be decreased except for the dihydroform reacting with alcohol dehydrogenase from liver.
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| Author: | Reinhard JeckGND, Christoph Woenckhaus, Antonin Holý |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-721419 |
| DOI: | https://doi.org/10.1515/znc-1975-11-1207 |
| ISSN: | 0939-5075 |
| ISSN: | 1865-7125 |
| Parent Title (German): | Zeitschrift für Naturforschung, C |
| Publisher: | Verlag der Zeitschrift für Naturforschung |
| Place of publication: | Tübingen |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2014/06/02 |
| Year of first Publication: | 1975 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2024/01/30 |
| Tag: | Complex Formation; Dehydrogenase; NAD⊕-Isomer |
| Volume: | 30.1975 |
| Issue: | 11-12 |
| Page Number: | 5 |
| First Page: | 734 |
| Last Page: | 738 |
| Institutes: | Medizin |
| Biochemie, Chemie und Pharmazie / Biochemie und Chemie | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0 |
