WormRuler: a software to track body length used to characterize a super red-shifted channelrhodopsin in Caenorhabditis elegans

  • Manipulation of neuronal or muscular activity by optogenetics or other stimuli can be directly linked to the analysis of Caenorhabditis elegans (C. elegans) body length. Thus, WormRuler was developed as an open-source video analysis toolbox that offers video processing and data analysis in one application. Utilizing this novel tool, the super red-shifted channelrhodopsin variant, ChrimsonSA, was characterized in C. elegans. Expression and activation of ChrimsonSA in GABAergic motor neurons results in their depolarization and therefore elongation of body length, the extent of which providing information about the strength of neuronal transmission.

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Author:Marius SeidenthalORCiD, Dennis VettkötterORCiD, Alexander GottschalkORCiDGND
URN:urn:nbn:de:hebis:30:3-752211
DOI:https://doi.org/10.17912/micropub.biology.000607
ISSN:2578-9430
Parent Title (English):MicroPublication
Publisher:Caltech Library
Place of publication:Pasadena, CA
Document Type:Article
Language:English
Date of Publication (online):2022/07/19
Date of first Publication:2022/07/19
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2024/08/28
Volume:2022
Page Number:5
Note:
Gefördert durch den Open-Access-Publikationsfonds der Goethe-Universität
Note:
Funding: DFG ; CRC1080/B2
Note:
Funding: DFG ; GO1011/12-2
HeBIS-PPN:52120626X
Institutes:Biochemie, Chemie und Pharmazie
Fachübergreifende Einrichtungen / Buchmann Institut für Molekulare Lebenswissenschaften (BMLS)
Dewey Decimal Classification:0 Informatik, Informationswissenschaft, allgemeine Werke / 00 Informatik, Wissen, Systeme / 004 Datenverarbeitung; Informatik
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Open-Access-Publikationsfonds:Biochemie, Chemie und Pharmazie
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International