New insights into RNA processing by the eukaryotic tRNA splicing endonuclease
- Through its role in intron cleavage, tRNA splicing endonuclease (TSEN) plays a critical function in the maturation of intron-containing pre-tRNAs. The catalytic mechanism and core requirement for this process is conserved between archaea and eukaryotes, but for decades, it has been known that eukaryotic TSENs have evolved additional modes of RNA recognition, which have remained poorly understood. Recent research identified new roles for eukaryotic TSEN, including processing or degradation of additional RNA substrates, and determined the first structures of pre-tRNA-bound human TSEN complexes. These recent discoveries have changed our understanding of how the eukaryotic TSEN targets and recognizes substrates. Here, we review these recent discoveries, their implications, and the new questions raised by these findings.
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| Author: | Cassandra K. HayneORCiD, Samoil SekulovskiORCiD, Jennifer E. HurtigORCiD, Robin E. StanleyORCiD, Simon TrowitzschORCiDGND, Ambro van HoofORCiD |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-771086 |
| DOI: | https://doi.org/10.1016/j.jbc.2023.105138 |
| ISSN: | 0021-9258 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/37544645 |
| Parent Title (English): | Journal of biological chemistry |
| Publisher: | American Society for Biochemistry and Molecular Biology Publications |
| Place of publication: | Bethesda, Md |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2021/01/04 |
| Date of first Publication: | 2023/08/05 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2024/04/23 |
| Tag: | RNA; RNA degradation; RNA processing; TSEN; protein structure; tRNA splicing |
| Volume: | 299.2023 |
| Issue: | 9 |
| Page Number: | 1 |
| First Page: | 105138 |
| Last Page: | 105138 |
| Institutes: | Biochemie, Chemie und Pharmazie |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - CC BY - Namensnennung 4.0 International |
