New insights into RNA processing by the eukaryotic tRNA splicing endonuclease

  • Through its role in intron cleavage, tRNA splicing endonuclease (TSEN) plays a critical function in the maturation of intron-containing pre-tRNAs. The catalytic mechanism and core requirement for this process is conserved between archaea and eukaryotes, but for decades, it has been known that eukaryotic TSENs have evolved additional modes of RNA recognition, which have remained poorly understood. Recent research identified new roles for eukaryotic TSEN, including processing or degradation of additional RNA substrates, and determined the first structures of pre-tRNA-bound human TSEN complexes. These recent discoveries have changed our understanding of how the eukaryotic TSEN targets and recognizes substrates. Here, we review these recent discoveries, their implications, and the new questions raised by these findings.

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Author:Cassandra K. HayneORCiD, Samoil SekulovskiORCiDGND, Jennifer E. HurtigORCiD, Robin E. StanleyORCiD, Simon TrowitzschORCiDGND, Ambro van HoofORCiD
URN:urn:nbn:de:hebis:30:3-771086
DOI:https://doi.org/10.1016/j.jbc.2023.105138
ISSN:0021-9258
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/37544645
Parent Title (English):Journal of biological chemistry
Publisher:American Society for Biochemistry and Molecular Biology Publications
Place of publication:Bethesda, Md
Document Type:Article
Language:English
Date of Publication (online):2021/01/04
Date of first Publication:2023/08/05
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2024/04/23
Tag:RNA; RNA degradation; RNA processing; TSEN; protein structure; tRNA splicing
Volume:299.2023
Issue:9
Page Number:1
First Page:105138
Last Page:105138
Institutes:Biochemie, Chemie und Pharmazie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International