How to extract and analyze pollen from internal organs and exoskeletons of fossil insects
- Highlights • Protocol for extracting and analyzing pollen grains from fossil insects • Individual fossil grains can be analyzed using a combined approach • Simple and fast TEM embedding and sectioning protocol • Protocol enables a taxonomic assignment of pollen Summary This protocol explains how to extract pollen from fossil insects with subsequent descriptions of pollen treatment. We also describe how to document morphological and ultrastructural features with light-microscopy and electron microscopy. It enables a taxonomic assignment of pollen that can be used to interpret flower-insect interactions, foraging and feeding behavior of insects, and the paleoenvironment. The protocol is limited by the state of the fossil, the presence/absence of pollen on fossil specimens, and the availability of extant pollen for comparison.
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| Author: | Friðgeir GrímssonORCiD, Silvia UlrichORCiDGND, Reinhard ZetterORCiD, Thomas HörnschemeyerORCiDGND, Michael S. EngelORCiDGND, Sonja WedmannORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-781821 |
| DOI: | https://doi.org/10.1016/j.xpro.2021.100923 |
| ISSN: | 2666-1667 |
| Parent Title (English): | STAR Protocols |
| Publisher: | Elsevier |
| Place of publication: | Amsterdam |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2021/10/29 |
| Date of first Publication: | 2021/10/29 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2024/03/26 |
| Tag: | Earth sciences; Evolutionary biology; Microscopy; Plant sciences |
| Volume: | 2.2021 |
| Issue: | 100923 |
| Article Number: | 100923 |
| Page Number: | 20 |
| First Page: | 1 |
| Last Page: | 19 |
| HeBIS-PPN: | 52098255X |
| Institutes: | Biowissenschaften |
| Angeschlossene und kooperierende Institutionen / Senckenbergische Naturforschende Gesellschaft | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 56 Fossilien, Paläontologie / 560 Paläontologie; Paläozoologie |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - CC BY - Namensnennung 4.0 International |
