Getting more out of the zebrafish light dark transition test

  • In (eco-)toxicological studies the light/dark transition (LDT) test is one of the most frequently used behaviour assays with zebrafish eleutheroembryos. However, study results vary regarding data presentation and analysis and mostly focus on a limited amount of the recorded data. In this study, we investigated whether monitoring two behavioural outcomes (time and distance moved) together with analysing multiple parameters can improve test sensitivity and data interpretation. As a proof of principle 5-day old zebrafish (Danio rerio) eleutheroembryos exposed to either endocrine disruptors (EDs) or acetylcholine esterase (AChE) inhibitors were investigated. We analysed conventional parameters such as mean and sum and implemented additional endpoints such as minimum or maximum distance moved and new parameters assessing the bursting response of eleutheroembryos. Furthermore, changes in eleutheroembryonic behaviour during the moment of the light to dark transition were added. To improve data presentation control-normalised results were displayed in radar charts, enabling the simultaneous presentation of different parameters in relation to each other. This enabled us to identify parameters most relevant to a certain behavioural response. A cut off threshold using control data was applied to identify parameters that were altered in a biological relevant manner. Our approach was able to detect effects on different parameters that remained undetected when analysis was done using conventional bar graphs on - in most cases analysed - averaged, mean distance moved values. By combining the radar charts with additional parameters and by using control-based thresholds, we were able to increase the test sensitivity and promote a deeper understanding of the behaviour response of zebrafish eleutheroembryos in the LDT test and thereby increased its usability for behavioural toxicity studies.
Metadaten
Author:Ann-Cathrin HaigisORCiDGND, Richard OttermannsORCiDGND, Andreas Herbert SchiwyORCiDGND, Henner HollertORCiDGND, Jessica LegradiORCiDGND
URN:urn:nbn:de:hebis:30:3-784320
DOI:https://doi.org/10.1016/j.chemosphere.2022.133863
ISSN:1879-1298
Parent Title (English):Chemosphere
Publisher:Elsevier Science
Place of publication:Amsterdam [u.a.]
Document Type:Article
Language:English
Date of Publication (online):2022/02/03
Date of first Publication:2022/02/03
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2024/05/27
Tag:Behaviour; Endocrine disruptors; Light dark transition test; Organophosphates; Zebrafish eleutheroembryo
Volume:295
Issue:art. 133863
Article Number:133863
Page Number:11
First Page:1
Last Page:11
Note:
The authors thank the German federal ministry of education and science BMBF (Project number 02WRS1419C) for their support.
HeBIS-PPN:520908627
Institutes:Biowissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International