Isolation and characterization of mutants of saccharomyces cerevisiae auxotrophic and conditionally auxotrophic for 5′-dTMP
- An improved method for isolation of yeast m utants auxotrophic for 5′-dTM P is presented. The procedure employs the two folic acid antagonists am inopterin and sulfanilam ide (SAA). Selectiveness of the procedure depends on concentration of SAA and time of incubation. 44 mutants auxotrophic and 3 conditionally auxotrophic for 5′-dTMP were isolated. All belong to one complementation group. The corresponding gene was designated TMP1. Tetrad dissection revealed its chromosomal nature. TMP1 is not closely linked to the genes ADE2,, LEU1, ARG 4, ILV2, HIS5, LYS1 and the mating type locus. With the centromere-linked genes ARG4 and LEU1 I gene TMP1 exhibited second division segregation frequencies of 0.42 and 0.53 respectively, indicative of centromere-linkage. Strains auxotrophic and conditionally auxotrophic for 5′-dTM P were all respiratory deficient (petite). Genetical analysis indicates that the petite phenotype is due to loss of the rho factor in cells harbouring either tmp1 or tmp1ts alleles.
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| Author: | Martin BrendelORCiD, Wolfgang Walter Fäth |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-811837 |
| DOI: | https://doi.org/10.1515/znc-1974-11-1214 |
| ISSN: | 0939-5075 |
| ISSN: | 1865-7125 |
| Parent Title (German): | Zeitschrift für Naturforschung, C |
| Publisher: | Verlag der Zeitschrift für Naturforschung |
| Place of publication: | Tübingen |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2014/06/02 |
| Year of first Publication: | 1974 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2024/02/02 |
| Tag: | Elimination of Rho Factor; Folic Acid Antagonists; Tetrad Analysis; Thymidylate Synthetase |
| Volume: | 29.1974 |
| Issue: | 11-12 |
| Page Number: | 8 |
| First Page: | 733 |
| Last Page: | 738 |
| Institutes: | Biowissenschaften |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0 |
