Hippocampal cytokine release in experimental epileptogenesis - a longitudinal in vivo microdialysis study
- Background: Inflammation, particularly cytokine release, contributes to epileptogenesis by influencing the cerebral tissue remodeling and neuronal excitability that occurs after a precipitating epileptogenic insult. While several cytokines have been explored in this process, release kinetics are less well investigated. Determining the time course of cytokine release in the epileptogenic zone is necessary for precisely timed preventive or therapeutic anti-inflammatory interventions. Methods: Hippocampal extracellular levels of six cytokines and chemokines (IL-1β, IL-6, IL-10, CCL2, CCL3, and CCL5) were quantified at various time points during epileptogenesis in a rat model of mesial temporal lobe epilepsy with hippocampal sclerosis (mTLE-HS) using microdialysis (MD). Results: The analysis of microdialysates demonstrated consistent elevation at all time points during epileptogenesis for IL-1β and IL-10. IL-10 release was maximal on day 1, IL-1β release peaked at day 8. No correlation between local hippocampal IL-1β concentrations and IL-1β blood levels was found. Conclusion: The release kinetics of IL-1β are consistent with its established pro-epileptogenic properties, while the kinetics of IL-10 suggest a counter-regulatory effect. This proof-of-concept study demonstrates the feasibility of intraindividual longitudinal monitoring of hippocampal molecular inflammatory processes via repetitive MD over several weeks and sheds light on the kinetics of hippocampal cytokine release during epileptogenesis.
Author: | Kai SiebenbrodtORCiDGND, Vanessa Schütz, Lara Sophie CostardORCiD, Valentin Neubert, Daniel Alvarez-FischerGND, Kerstin SeidelORCiD, Bernd SchmeckORCiDGND, Sven MeuthORCiDGND, Felix RosenowORCiDGND, Sebastian BauerORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-823748 |
DOI: | https://doi.org/10.3390/brainsci12050677 |
ISSN: | 2076-3425 |
Parent Title (English): | Brain Sciences |
Publisher: | MDPI |
Place of publication: | Basel |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2022/05/21 |
Date of first Publication: | 2022/05/21 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2024/02/22 |
Tag: | epilepsy; hippocampal sclerosis; inflammation; rat model; temporal lobe epilepsy |
Volume: | 12 |
Issue: | 5, art. 677 |
Article Number: | 677 |
Page Number: | 12 |
First Page: | 1 |
Last Page: | 12 |
Note: | The authors were supported by the LOEWE Center for Personalized Translational Epilepsy Research (CePTER), Goethe University, Frankfurt am Main, Germany, funded by the Hessen State Ministry for Higher Education, Research and the Arts (HMWK). |
HeBIS-PPN: | 517871599 |
Institutes: | Medizin |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |