Cyclin CLB2 mRNA localization and protein synthesis link cell cycle progression to bud growth

  • Clb2 is a conserved mitotic B-type cyclin, the levels of which are finely controlled to drive progression through the cell cycle. While it is known that CLB2 transcription and Clb2 protein degradation are important for precise control of its expression, it remains unclear whether the synthesis of Clb2 is also regulated. To address whether and how Clb2 expression levels respond to cell growth changes and adapt cell cycle progression, we combined single-cell and single-molecule imaging methods to measure CLB2 mRNA and protein expression throughout the Saccharomyces cerevisiae cell cycle. We found that the CLB2 mRNA was efficiently localized to the yeast bud as soon as this compartment was formed, but strikingly the Clb2 protein accumulated in the mother nucleus. The CLB2 mRNA localization in the yeast bud by the She2-3 complex did not control protein localization but rather promoted CLB2 translation. Moreover, CLB2 mRNA bud localization and protein synthesis were coupled and dependent on a single secondary structure -a ZIP code-located in the coding sequence. In a CLB2 ZIP code mutant, mRNA localization was impaired and Clb2 protein synthesis decreased, resulting in changes in cell cycle distribution and increased size of daughter cells at birth. Finally, while in WT cells the Clb2 protein concentration followed bud growth, this relationship was impaired in the ZIP code mutant. We propose that S. cerevisiae couples the control of CLB2 mRNA bud localization and protein synthesis to coordinate cell growth and cell cycle progression. This mechanism extends our knowledge of CLB2 expression regulation, and constitutes a novel function for mRNA localization.

Export metadata

Additional Services

Share in Twitter Search Google Scholar
Metadaten
Author:Anna MaekiniemiORCiD, Philipp SavakisORCiD, Jacky L. SnoepORCiDGND, Markus SeilerORCiDGND, Kelly van RossumORCiD, David D. van Niekerk, Katharina ZarnackORCiDGND, Robert H. SingerORCiD, Evelina TutucciORCiD
URN:urn:nbn:de:hebis:30:3-860751
URL:https://www.biorxiv.org/content/10.1101/2022.03.01.481833v3
DOI:https://doi.org/10.1101/2022.03.01.481833
Parent Title (English):bioRxiv
Publisher:bioRxiv
Document Type:Preprint
Language:English
Date of Publication (online):2024/06/23
Date of first Publication:2024/06/23
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2024/07/03
Issue:2022.03.01.481833 Version 3
Edition:Version 3
Page Number:63
Institutes:Biowissenschaften / Biowissenschaften
Fachübergreifende Einrichtungen / Buchmann Institut für Molekulare Lebenswissenschaften (BMLS)
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International