TY  - JOUR
A1  - Rosenbaum, Florian P.
A1  - Poehlein, Anja
A1  - Daniel, Rolf
A1  - Müller, Volker
T1  - Energy-conserving dimethyl sulfoxide reduction in the acetogenic bacterium Moorella thermoacetica
T2  - Environmental microbiology
N2  - Moorella thermoacetica is one of the well-studied thermophilic acetogenic bacteria. It grows by oxidation of organic substrates, CO or H2 coupled to CO2 reduction to acetate. Here, we describe that M. thermoacetica can also use dimethyl sulfoxide as terminal electron acceptor. Growth of M. thermoacetica on glucose or H2 + CO2 was stimulated by dimethyl sulfoxide (DMSO). Membranes showed a DMSO reductase activity, that was induced by growing cells in presence of DMSO. The enzyme used reduced anthraquinone-2,6-disulfonate, benzyl- and methyl viologen as electron donor, but not NAD(P)H. Activity was highest at pH 5 and 60°C, the Km for DMSO was 2.4 mM. Potential DMSO reductase subunits were identified by peptide mass fingerprinting; they are encoded in a genomic region that contains three potential dmsA genes, three dmsB genes and one dmsC gene. Transcriptome analysis revealed that two different dmsAB gene clusters were induced in the presence of DMSO. The function of these two and their predicted biochemical features are discussed. In addition, the data are in line with the hypothesis that M. thermoacetica can use DMSO alongside CO2 as electron acceptor and DMSO reduction is catalysed by an energy-conserving, membrane-bound electron transport chain with DMSO as final electron acceptor.
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/76122
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-761222
SN  - 1462-2920
N1  - This work was funded by the Deutsche Forschungsgemeinschaft.
N1  - Transcriptome data have been deposited in the National Center for Biotechnology Information's (NCBI) Sequence Read Archive (SRA) under accession no. SRR18189544 – SRR18189549.
VL  - 24
IS  - 4
SP  - 2000
EP  - 2012
PB  - Blackwell
CY  - Oxford [u.a.]
ER  -