TY  - JOUR
A1  - Lopalco, Patrizia
A1  - Stahl, Julia
A1  - Annese, Cosimo
A1  - Averhoff, Beate
A1  - Corcelli, Angela
T1  - Identification of unique cardiolipin and monolysocardiolipin species in Acinetobacter baumannii
T2  - Scientific Reports
N2  - Acidic glycerophospholipids play an important role in determining the resistance of Gram-negative bacteria to stress conditions and antibiotics. Acinetobacter baumannii, an opportunistic human pathogen which is responsible for an increasing number of nosocomial infections, exhibits broad antibiotic resistances. Here lipids of A. baumannii have been analyzed by combined MALDI-TOF/MS and TLC analyses; in addition GC-MS analyses of fatty acid methyl esters released by methanolysis of membrane phospholipids have been performed. The main glycerophospholipids are phosphatidylethanolamine, phosphatidylglycerol, acyl-phosphatidylglycerol and cardiolipin together with monolysocardiolipin, a lysophospholipid only rarely detected in bacterial membranes. The major acyl chains in the phospholipids are C16:0 and C18:1, plus minor amounts of short chain fatty acids. The structures of the cardiolipin and monolysocardiolipin have been elucidated by post source decay mass spectrometry analysis. A large variety of cardiolipin and monolysocardiolipin species were found in A. baumannii. Similar lysocardiolipin levels were found in the two clinical strains A. baumannii ATCC19606T and AYE whereas in the nonpathogenic strain Acinetobacter baylyi ADP1 lysocardiolipin levels were highly reduced.
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/82758
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-827581
SN  - 2045-2322
VL  - 7
IS  - Article number: 2972
PB  - Macmillan Publishers Limited, part of Springer Nature
CY  - London
ER  -