TY  - JOUR
A1  - Pfaff, Julia
A1  - Reinwald, Hannes
A1  - Ayobahan, Steve U.
A1  - Alvincz, Julia
A1  - Göckener, Bernd
A1  - Shomroni, Orr
A1  - Salinas-Riester, Gabriela
A1  - Düring, Rolf-Alexander
A1  - Schäfers, Christoph
A1  - Eilebrecht, Sebastian
T1  - Toxicogenomic differentiation of functional responses to fipronil and imidacloprid in Daphnia magna
T2  - Aquatic Toxicology
N2  - Active substances of pesticides, biocides or pharmaceuticals can induce adverse side effects in the aquatic ecosystem, necessitating environmental hazard and risk assessment prior to substance registration. The freshwater crustacean Daphnia magna is a model organism for acute and chronic toxicity assessment representing aquatic invertebrates. However, standardized tests involving daphnia are restricted to the endpoints immobility and reproduction and thus provide only limited insights into the underlying modes-of-action. Here, we applied transcriptome profiling to a modified D. magna Acute Immobilization test to analyze and compare gene expression profiles induced by the GABA-gated chloride channel blocker fipronil and the nicotinic acetylcholine receptor (nAChR) agonist imidacloprid. Daphnids were expose to two low effect concentrations of each substance followed by RNA sequencing and functional classification of affected gene ontologies and pathways. For both insecticides, we observed a concentration-dependent increase in the number of differentially expressed genes, whose expression changes were highly significantly positively correlated when comparing both test concentrations. These gene expression fingerprints showed virtually no overlap between the test substances and they related well to previous data of diazepam and carbaryl, two substances targeting similar molecular key events. While, based on our results, fipronil predominantly interfered with molecular functions involved in ATPase-coupled transmembrane transport and transcription regulation, imidacloprid primarily affected oxidase and oxidoreductase activity. These findings provide evidence that systems biology approaches can be utilized to identify and differentiate modes-of-action of chemical stressors in D. magna as an invertebrate aquatic non-target organism. The mechanistic knowledge extracted from such data will in future contribute to the development of Adverse Outcome Pathways (AOPs) for read-across and prediction of population effects.
KW  - Ecotoxicogenomics
KW  - D. magna
KW  - Neurotoxicity
KW  - Pathways
KW  - Biomarkers
KW  - Pesticides
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63068
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-630687
SN  - 1879-1514
N1  - This work was supported by the Fraunhofer Internal Programs under Grant No. Attract 040-600300.
VL  - 238
IS  - art. 105927
SP  - 1
EP  - 11
PB  - Elsevier Science
CY  - Amsterdam [u.a.]
ER  -