TY  - INPR
A1  - Yin, Max Dongsheng
A1  - Lemaire, Olivier N.
A1  - Rosas-Jiménez, José Guadalupe
A1  - Belhamri, Mélissa
A1  - Shevchenko, Anna
A1  - Hummer, Gerhard
A1  - Wagner, Tristan
A1  - Murphy, Bonnie J.
T1  - Snapshots of acetyl-CoA synthesis, the final step of CO₂ fixation in the Wood-Ljungdahl pathway
T2  - bioRxiv
N2  - In the ancient microbial Wood-Ljungdahl pathway, CO2 is fixed in a multi-step process with acetyl-CoA synthesis at the bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase complex (CODH/ACS). Here, we present catalytic snapshots of the CODH/ACS from the gas-converting acetogen Clostridium autoethanogenum, characterizing the molecular choreography of the overall reaction including electron transfer to the CODH for CO2 reduction, methyl transfer from the corrinoid iron-sulfur protein (CoFeSP) partner to the ACS active site and acetyl-CoA production. Unlike CODH, the multidomain ACS undergoes large conformational changes to form an internal connection to the CODH active site, accommodate the CoFeSP for methyl transfer and protect the reaction intermediates. Altogether, the structures allow us to draw a detailed reaction mechanism of this enzyme crucial for CO2 fixation in anaerobic organisms.
Y1  - 2024
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/86498
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-864981
UR  - https://www.biorxiv.org/content/10.1101/2024.08.05.606187v1
IS  - 2024.08.05.606187v1
PB  - bioRxiv
ER  -