TY  - JOUR
A1  - Horst, Klemens
A1  - Eschbach, Daphne-Asimenia
A1  - Pfeifer, Roman
A1  - Relja, Borna
A1  - Sassen, Martin
A1  - Steinfeldt, Thorsten
A1  - Wulf, Hinnerk
A1  - Vogt, Nina
A1  - Frink, Michael
A1  - Ruchholtz, Steffen
A1  - Pape, Hans-Christoph
A1  - Hildebrand, Frank
T1  - Long-term effects of induced hypothermia on local and systemic inflammation - results from a porcine long-term trauma model
T2  - PLoS One
N2  - Background: Hypothermia has been discussed as playing a role in improving the early phase of systemic inflammation. However, information on the impact of hypothermia on the local inflammatory response is sparse. We therefore investigated the kinetics of local and systemic inflammation in the late posttraumatic phase after induction of hypothermia in an established porcine long-term model of combined trauma.
Materials & Methods: Male pigs (35 ± 5kg) were mechanically ventilated and monitored over the study period of 48 h. Combined trauma included tibia fracture, lung contusion, liver laceration and pressure-controlled hemorrhagic shock (MAP < 30 ± 5 mmHg for 90 min). After resuscitation, hypothermia (33°C) was induced for a period of 12 h (HT-T group) with subsequent re-warming over a period of 10 h. The NT-T group was kept normothermic. Systemic and local (fracture hematoma) cytokine levels (IL-6, -8, -10) and alarmins (HMGB1, HSP70) were measured via ELISA.
Results: Severe signs of shock as well as systemic and local increases of pro-inflammatory mediators were observed in both trauma groups. In general the local increase of pro- and anti-inflammatory mediator levels was significantly higher and prolonged compared to systemic concentrations. Induction of hypothermia resulted in a significantly prolonged elevation of both systemic and local HMGB1 levels at 48 h compared to the NT-T group. Correspondingly, local IL-6 levels demonstrated a significantly prolonged increase in the HT-T group at 48 h.
Conclusion: A prolonged inflammatory response might reduce the well-described protective effects on organ and immune function observed in the early phase after hypothermia induction. Furthermore, local immune response also seems to be affected. Future studies should aim to investigate the use of therapeutic hypothermia at different degrees and duration of application.
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/41503
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-415039
SN  - 1932-6203
N1  - Copyright: © 2016 Horst et al. This is an open access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 11
IS  - (5): e0154788
SP  - 1
EP  - 15
PB  - PLoS
CY  - Lawrence, Kan.
ER  -