TY  - JOUR
A1  - Duchardt-Ferner, Elke
A1  - Nilsson, Lennart
A1  - Schleucher, Jürgen
T1  - Cytosine ribose flexibility in DNA : a combined NMR 13C spin relaxation and molecular dynamics simulation study
T2  - Nucleic acids research
N2  - Using (13)C spin relaxation NMR in combination with molecular dynamic (MD) simulations, we characterized internal motions within double-stranded DNA on the pico- to nano-second time scale. We found that the C-H vectors in all cytosine ribose moieties within the Dickerson-Drew dodecamer (5´-CGCGAATTCGCG-3´) are subject to high amplitude motions, while the other nucleotides are essentially rigid. MD simulations showed that repuckering is a likely motional model for the cytosine ribose moiety. Repuckering occurs with a time constant of around 100 ps. Knowledge of DNA dynamics will contribute to our understanding of the recognition specificity of DNA-binding proteins such as cytosine methyltransferase.
Y1  - 2008
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/6015
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-60285
SN  - 1362-4962
SN  - 0305-1048
N1  - This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 36
IS  - 12
SP  - 4211
EP  - 4219
PB  - Oxford Univ. Press
CY  - Oxford
ER  -