TY  - JOUR
A1  - Pantazopoulou, Vasiliki I.
A1  - Delis, Anastasios D.
A1  - Georgiou, Stella
A1  - Pagakis, Stamatis N.
A1  - Filippa, Vicky
A1  - Dragona, Eleni
A1  - Kloukina, Ismini
A1  - Chatzitheodoridis, Elias
A1  - Trebicka, Jonel
A1  - Velentzas, Athanassios D.
A1  - Thiele, Maja
A1  - Gagos, Sarantis
A1  - Thanos, Dimitris
A1  - Tseleni-Balafouta, Sofia
A1  - Stravopodis, Dimitrios J.
A1  - Anastasiadou, Ema
T1  - AGO2 localizes to cytokinetic protrusions in a p38-dependent manner and is needed for accurate cell division
T2  - Communications biology
N2  - Argonaute 2 (AGO2) is an indispensable component of the RNA-induced silencing complex, operating at the translational or posttranscriptional level. It is compartmentalized into structures such as GW- and P-bodies, stress granules and adherens junctions as well as the midbody. Here we show using immunofluorescence, image and bioinformatic analysis and cytogenetics that AGO2 also resides in membrane protrusions such as open- and close-ended tubes. The latter are cytokinetic bridges where AGO2 colocalizes at the midbody arms with cytoskeletal components such as α-Τubulin and Aurora B, and various kinases. AGO2, phosphorylated on serine 387, is located together with Dicer at the midbody ring in a manner dependent on p38 MAPK activity. We further show that AGO2 is stress sensitive and important to ensure the proper chromosome segregation and cytokinetic fidelity. We suggest that AGO2 is part of a regulatory mechanism triggered by cytokinetic stress to generate the appropriate micro-environment for local transcript homeostasis.
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63280
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-632808
SN  - 2399-3642
N1  - This work has been funded in part by the European Union’s Horizon 2020 Research and Innovation Program under Grant Agreement no 668031 (GALAXY: Gut-and-Liver Axis in Alcoholic Liver Fibrosis) and by Greece and the European Union (European Social Fund-ESF) through Operational Programme “Human Resources Development, Education and Lifelong Learning” in the context of the project “Strengthening Human Resources Research Potential via Doctorate Research” (MIS-5000432), implemented by the State Scholarships Foundation (IKY).
VL  - 4
IS  - art. 726
SP  - 1
EP  - 18
PB  - Springer Nature
CY  - London
ER  -