TY  - JOUR
A1  - Sastre, Enrique Andrés
A1  - Zaucke, Frank
A1  - Witte-Bouma, Janneke
A1  - Osch, Geertruda Johanna Victoria Maria van
A1  - Farrell, Eric
T1  - Cartilage oligomeric matrix protein-derived peptides secreted by cartilage do not induce responses commonly observed during osteoarthritis
T2  - Cartilage
N2  - Objective: To evaluate if 3 peptides derived from the cartilage oligomeric matrix protein (COMP), which wounded zones of cartilage secrete into synovial fluid, possess biological activity and might therefore be involved in the regulation of specific aspects of joint regeneration.
Methods: The 3 peptides were produced by chemical synthesis and then tested in vitro for known functions of the COMP C-terminal domain from which they derive, and which are involved in osteoarthritis: transforming growth factor-β (TGF-β) signaling, vascular homeostasis, and inflammation. Results. None of the peptides affected the gene expression of COMP in osteochondral progenitor cells (P > 0.05). We observed no effects on the vascularization potential of endothelial cells (P > 0.05). In cultured synovium explants, no differences on the expression of catabolic enzymes or proinflammatory cytokines were found when peptides were added (P > 0.05).
Discussion and conclusions: The 3 peptides tested do not regulate TGF-β signaling, angiogenesis and vascular tube formation, or synovial inflammation in vitro and therefore most likely do not play a major role in the disease process.
KW  - cartilage oligomeric matrix protein
KW  - endothelial cells
KW  - matrikine
KW  - synovium
KW  - transforming growth factor-β
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/55997
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-559979
SN  - 1947-6043
VL  - 2020
IS  - September
PB  - SAGE Publications
CY  - London
ER  -