TY  - JOUR
A1  - Leo, Felix
A1  - Schwarz, Fabian M.
A1  - Schuchmann, Kai
A1  - Müller, Volker
T1  - Capture of carbon dioxide and hydrogen by engineered Escherichia coli: hydrogen-dependent CO₂ reduction to formate
T2  - Applied microbiology and biotechnology
N2  - In times of global climate change and the fear of dwindling resources, we are facing different considerable challenges such as the replacement of fossil fuel–based energy carriers with the coincident maintenance of the increasing energy supply of our growing world population. Therefore, CO2 capturing and H2 storing solutions are urgently needed. In this study, we demonstrate the production of a functional and biotechnological interesting enzyme complex from acetogenic bacteria, the hydrogen-dependent CO2 reductase (HDCR), in the well-known model organism Escherichia coli. We identified the metabolic bottlenecks of the host organisms for the production of the HDCR enzyme complex. Here we show that the recombinant expression of a heterologous enzyme complex transforms E. coli into a whole-cell biocatalyst for hydrogen-driven CO2 reduction to formate without the need of any external co-factors or endogenous enzymes in the reaction process. This shifts the industrial platform organism E. coli more and more into the focus as biocatalyst for CO2-capturing and H2-storage. Key points: A functional HDCR enzyme complex was heterologously produced in E. coli; The metabolic bottlenecks for HDCR production were identified; HDCR enabled E. coli cell to capture and store H2 and CO2 in the form of formate.
KW  - Biocatalysis
KW  - Heterologous enzyme production
KW  - Hydrogen-dependent CO2 reductase
KW  - Acetobacterium woodii
KW  - CO2 capture
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62602
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-626024
SN  - 1432-0614
N1  - Open access funding enabled and organized by Projekt DEAL. This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement number 741791).
VL  - 105
SP  - 5861
EP  - 5872
PB  - Springer
CY  - Berlin; Heidelberg; New York
ER  -