TY  - JOUR
A1  - Pöschel, Laura
A1  - Gehr, Elisabeth
A1  - Jordan, Paulina
A1  - Sonntag, Frank
A1  - Buchhaupt, Markus
T1  - Expression of toxic genes in Methylorubrum extorquens with a tightly repressed, cumate-inducible promoter
T2  - Antonie van Leeuwenhoek
N2  - Methylorubrum extorquens is an important model methylotroph and has enormous potential for the development of C1-based microbial cell factories. During strain construction, regulated promoters with a low background expression level are important genetic tools for expression of potentially toxic genes. Here we present an accordingly optimised promoter, which can be used for that purpose. During construction and testing of terpene production strains harbouring a recombinant mevalonate pathway, strong growth defects were observed which made strain development impossible. After isolation and characterisation of suppressor mutants, we discovered a variant of the cumate-inducible promoter PQ2148 used in this approach. Deletion of 28 nucleotides resulted in an extremely low background expression level, but also reduced the maximal expression strength to about 30% of the original promoter. This tightly repressed promoter version is a powerful module for controlled expression of potentially toxic genes in M. extorquens.
KW  - Inducible Promoter
KW  - Methylorubrum extorquens AM1
KW  - Alphaproteobacteria
KW  - Cumate
KW  - Background expression
Y1  - 2023
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/79557
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-795575
SN  - 1572-9699
VL  - 2023
PB  - Springer Science + Business Media B.V
CY  - Dordrecht [u.a.]
ER  -