TY  - JOUR
A1  - Hegelein, Andreas Jürgen
A1  - Müller, Diana
A1  - Größl, Sylvester
A1  - Göbel, Michael
A1  - Hengesbach, Martin
A1  - Schwalbe, Harald
T1  - Genetic code expansion facilitates position‐selective labeling of rna for biophysical studies
T2  - Chemistry - a European journal
N2  - Nature relies on reading and synthesizing the genetic code with high fidelity. Nucleic acid building blocks that are orthogonal to the canonical A‐T and G‐C base‐pairs are therefore uniquely suitable to facilitate position‐specific labeling of nucleic acids. Here, we employ the orthogonal kappa‐xanthosine‐base‐pair for in vitro transcription of labeled RNA. We devised an improved synthetic route to obtain the phosphoramidite of the deoxy‐version of the kappa nucleoside in solid phase synthesis. From this DNA template, we demonstrate the reliable incorporation of xanthosine during in vitro transcription. Using NMR spectroscopy, we show that xanthosine introduces only minor structural changes in an RNA helix. We furthermore synthesized a clickable 7‐deaza‐xanthosine, which allows to site‐specifically modify transcribed RNA molecules with fluorophores or other labels.
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/56507
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-565074
SN  - 1521-3765
VL  - 26.2020
SP  - 1800
EP  - 1810
PB  - Wiley-VCH
CY  - Weinheim
ER  -