TY  - JOUR
A1  - Gläsener, Stephanie
A1  - Jänke, Christine
A1  - Habener, Anika
A1  - Geffers, Robert
A1  - Hagendorff, Petra
A1  - Witzlau, Katrin
A1  - Imelmann, Esther
A1  - Krueger, Andreas
A1  - Meyer-Bahlburg, Almut
T1  - Decreased production of class-switched antibodies in neonatal B cells is associated with increased expression of miR-181b
T2  - PLoS one
N2  - The increased susceptibility to infections of neonates is caused by an immaturity of the immune system as a result of both qualitative and quantitative differences between neonatal and adult immune cells. With respect to B cells, neonatal antibody responses are known to be decreased. Accountable for this is an altered composition of the neonatal B cell compartment towards more immature B cells. However, it remains unclear whether the functionality of individual neonatal B cell subsets is altered as well. In the current study we therefore compared phenotypical and functional characteristics of corresponding neonatal and adult B cell subpopulations. No phenotypic differences could be identified with the exception of higher IgM expression in neonatal B cells. Functional analysis revealed differences in proliferation, survival, and B cell receptor signaling. Most importantly, neonatal B cells showed severely impaired class-switch recombination (CSR) to IgG and IgA. This was associated with increased expression of miR-181b in neonatal B cells. Deficiency of miR-181b resulted in increased CSR. With this, our results highlight intrinsic differences that contribute to weaker B cell antibody responses in newborns.
KW  - B cells
KW  - MicroRNAs
KW  - Neonates
KW  - Flow cytometry
KW  - Blood
KW  - Adults
KW  - Antibodies
KW  - Cell staining
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/45642
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-456429
SN  - 1932-6203
N1  - Copyright: © 2018 Glaesener et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 13
IS  - (2): e0192230
SP  - 1
EP  - 23
PB  - PloS
CY  - Lawrence, Kan.
ER  -