TY  - JOUR
A1  - Harwardt, Marie-Lena I. E.
A1  - Schröder, Mark S.
A1  - Li, Yunqing
A1  - Malkusch, Sebastian
A1  - Freund, Petra
A1  - Gupta, Shashi
A1  - Janjic, Nebojsa
A1  - Strauss, Sebastian
A1  - Jungmann, Ralf
A1  - Dietz, Marina
A1  - Heilemann, Mike
T1  - Single-molecule super-resolution microscopy reveals heteromeric complexes of MET and EGFR upon ligand activation
T2  - International journal of molecular sciences
N2  - Receptor tyrosine kinases (RTKs) orchestrate cell motility and differentiation. Deregulated RTKs may promote cancer and are prime targets for specific inhibitors. Increasing evidence indicates that resistance to inhibitor treatment involves receptor cross-interactions circumventing inhibition of one RTK by activating alternative signaling pathways. Here, we used single-molecule super-resolution microscopy to simultaneously visualize single MET and epidermal growth factor receptor (EGFR) clusters in two cancer cell lines, HeLa and BT-20, in fixed and living cells. We found heteromeric receptor clusters of EGFR and MET in both cell types, promoted by ligand activation. Single-protein tracking experiments in living cells revealed that both MET and EGFR respond to their cognate as well as non-cognate ligands by slower diffusion. In summary, for the first time, we present static as well as dynamic evidence of the presence of heteromeric clusters of MET and EGFR on the cell membrane that correlates with the relative surface expression levels of the two receptors
KW  - receptor tyrosine kinases
KW  - MET
KW  - EGFR
KW  - receptor cross-interaction
KW  - single-molecule localization microscopy
KW  - single-particle tracking
KW  - DNA-PAINT
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/55320
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-553206
SN  - 1422-0067
SN  - 1661-6596
N1  - This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
VL  - 21
IS  - 8, art. 2803
SP  - 1
EP  - 20
PB  - MDPI
CY  - Basel
ER  -