TY  - INPR
A1  - Ali, Tamer
A1  - Rogala, Sandra
A1  - Krause, Nina M.
A1  - Bains, Jasleen Kaur
A1  - Melissari, Maria-Theodora
A1  - Währisch, Sandra
A1  - Schwalbe, Harald
A1  - Herrmann, Bernhard
A1  - Grote, Phillip
T1  - Fendrr synergizes with Wnt signalling to regulate fibrosis related genes during lung development via its RNA:dsDNA Triplex Element
T2  - bioRxiv
N2  - Long non-coding RNAs are a very versatile class of molecules that can have important roles in regulating a cells function, including regulating other genes on the transcriptional level. One of these mechanisms is that RNA can directly interact with DNA thereby recruiting additional components such as proteins to these sites via a RNA:dsDNA triplex formation. We genetically deleted the triplex forming sequence (FendrrBox) from the lncRNA Fendrr in mice and find that this FendrrBox is partially required for Fendrr function in vivo. We find that the loss of the triplex forming site in developing lungs causes a dysregulation of gene programs, associated with lung fibrosis. A set of these genes contain a triplex site directly at their promoter and are expressed in fibroblasts. We confirm the formation of RNA:dsDNA formation with target promoters. We find that Fendrr with the Wnt signalling pathway regulates these genes, implicating that Fendrr synergizes with Wnt signalling in lung fibrosis.
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72965
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-729659
UR  - https://www.biorxiv.org/content/10.1101/2021.11.02.466973v2
IS  - 2021.11.02.466973 Version 2
PB  - bioRxiv
ER  -