TY  - JOUR
A1  - Tombor, Lukas
A1  - John, David
A1  - Glaser, Simone F.
A1  - Luxán, Guillermo
A1  - Forte, Elvira
A1  - Furtado, Milena
A1  - Rosenthal, Nadia
A1  - Baumgarten, Nina
A1  - Schulz, Marcel Holger
A1  - Wittig, Janina
A1  - Rogg, Eva-Maria Pia
A1  - Manavski, Yosif
A1  - Fischer, Ariane
A1  - Muhly-Reinholz, Marion
A1  - Klee, Kathrin
A1  - Looso, Mario
A1  - Selignow, Carmen
A1  - Acker, Till
A1  - Bibli, Sofia Iris
A1  - Fleming, Ingrid
A1  - Patrick, Ralph
A1  - Harvey, Richard P.
A1  - Abplanalp, Wesley Tyler
A1  - Dimmeler, Stefanie
T1  - Single cell sequencing reveals endothelial plasticity with transient mesenchymal activation after myocardial infarction
T2  - Nature Communications
N2  - Endothelial cells play a critical role in the adaptation of tissues to injury. Tissue ischemia induced by infarction leads to profound changes in endothelial cell functions and can induce transition to a mesenchymal state. Here we explore the kinetics and individual cellular responses of endothelial cells after myocardial infarction by using single cell RNA sequencing. This study demonstrates a time dependent switch in endothelial cell proliferation and inflammation associated with transient changes in metabolic gene signatures. Trajectory analysis reveals that the majority of endothelial cells 3 to 7 days after myocardial infarction acquire a transient state, characterized by mesenchymal gene expression, which returns to baseline 14 days after injury. Lineage tracing, using the Cdh5-CreERT2;mT/mG mice followed by single cell RNA sequencing, confirms the transient mesenchymal transition and reveals additional hypoxic and inflammatory signatures of endothelial cells during early and late states after injury. These data suggest that endothelial cells undergo a transient mes-enchymal activation concomitant with a metabolic adaptation within the first days after myocardial infarction but do not acquire a long-term mesenchymal fate. This mesenchymal activation may facilitate endothelial cell migration and clonal expansion to regenerate the vascular network.
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/57745
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-577456
SN  - 2041-1723
VL  - 12
IS  - Article 681
PB  - Nature Publishing Group UK
CY  - [London]
ER  -