TY  - JOUR
A1  - Schimming, Olivia
A1  - Schmitt, Imke
A1  - Bode, Helge Björn
T1  - Identification of the Sfp-type PPTase EppA from the lichenized fungus Evernia prunastri
T2  - PLoS One
N2  - In the last decades, natural products from lichens have gained more interest for pharmaceutical application due to the broad range of their biological activity. However, isolation of the compounds of interest directly from the lichen is neither feasible nor sustainable due to slow growth of many lichens. In order to develop a pipeline for heterologous expression of lichen biosynthesis gene clusters and thus the sustainable production of their bioactive compounds we have identified and characterized the phosphopantheteinyl transferase (PPTase) EppA from the lichen Evernia prunastri. The Sfp-type PPTase EppA was functionally characterized through heterologous expression in E. coli using the production of the blue pigment indigoidine as readout and by complementation of a lys5 deletion in S. cerevisiae.
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/39043
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-390434
SN  - 1932-6203
N1  - Copyright: © 2016 Schimming et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 11
IS  - (1): e0145624
SP  - 1
EP  - 12
PB  - PLoS
CY  - Lawrence, Kan.
ER  -