TY  - JOUR
A1  - Müller, Diana
A1  - Bessi, Irene
A1  - Richter, Christian
A1  - Schwalbe, Harald
T1  - The folding landscapes of human telomeric RNA and DNA G-quadruplexes are markedly different
T2  - Angewandte Chemie
N2  - We investigated the folding kinetics of G-quadruplex (G4) structures by comparing the K+-induced folding of an RNA G4 derived from the human telomeric repeat-containing RNA (TERRA25) with a sequence homologous DNA G4 (wtTel25) using CD spectroscopy and real-time NMR spectroscopy. While DNA G4 folding is biphasic, reveals kinetic partitioning and involves kinetically favoured off-pathway intermediates, RNA G4 folding is faster and monophasic. The differences in kinetics are correlated to the differences in the folded conformations of RNA vs. DNA G4s, in particular with regard to the conformation around the glycosidic torsion angle χ that uniformly adopts anti conformations for RNA G4s and both, syn and anti conformation for DNA G4s. Modified DNA G4s with 19F bound to C2′ in arabino configuration adopt exclusively anti conformations for χ. These fluoro-modified DNA (antiTel25) reveal faster folding kinetics and monomorphic conformations similar to RNA G4s, suggesting the correlation between folding kinetics and pathways with differences in χ angle preferences in DNA and RNA, respectively.
KW  - folding landscapes
KW  - G-quadruplexes
KW  - kinetics
KW  - real-time NMR spectroscopy
KW  - TERRA RNA
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62102
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-621021
SN  - 1521-3773
N1  - Work at BMRZ is supported by the state of Hesse, D.M. has been supported by EU-funded iNEXT-discovery. H.S. and D.M. have been supported by DFG-Normalverfahren and by DFG collaborative research center 902. Open access funding enabled and organized by Projekt DEAL.
VL  - 60
IS  - 19
SP  - 10895
EP  - 10901
PB  - Wiley-VCH
CY  - Weinheim
ER  -