TY  - JOUR
A1  - Tascón, Igor
A1  - Sousa, Joana Sofia de
A1  - Corey, Robin A.
A1  - Mills, Deryck J.
A1  - Griwatz, David
A1  - Aumüller, Nadine
A1  - Mikusevic, Vedrana
A1  - Stansfeld, Phillip J.
A1  - Vonck, Janet
A1  - Hänelt, Inga
T1  - Structural basis of proton-coupled potassium transport in the KUP family
T2  - Nature Communications
N2  - Potassium homeostasis is vital for all organisms, but is challenging in single-celled organisms like bacteria and yeast and immobile organisms like plants that constantly need to adapt to changing external conditions. KUP transporters facilitate potassium uptake by the co-transport of protons. Here, we uncover the molecular basis for transport in this widely distributed family. We identify the potassium importer KimA from Bacillus subtilis as a member of the KUP family, demonstrate that it functions as a K+/H+ symporter and report a 3.7 Å cryo-EM structure of the KimA homodimer in an inward-occluded, trans-inhibited conformation. By introducing point mutations, we identify key residues for potassium and proton binding, which are conserved among other KUP proteins.
KW  - Bacterial structural biology
KW  - Cryoelectron microscopy
KW  - Ion transport
KW  - Permeation and transport
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/52882
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-528829
SN  - 2041-1723
N1  - Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
VL  - 11
IS  - 1, Art. 626
SP  - 1
EP  - 10
PB  - Nature Publishing Group UK
CY  - [London]
ER  -