TY  - JOUR
A1  - Winkelmann, Anne Ria
A1  - Schneider, Markus
A1  - Hartmann, Sylvia
A1  - Schnitzbauer, Andreas
A1  - Zeuzem, Stefan
A1  - Peveling-Oberhag, Jan Franz-Josef
A1  - Hansmann, Martin-Leo
A1  - Walter, Dirk Henning
T1  - Microsatellite instability occurs rarely in patients with Cholangiocarcinoma : a retrospective study from a German tertiary care hospital
T2  - International journal of molecular sciences
N2  - Immune-modulating therapy is a promising therapy for patients with cholangiocarcinoma (CCA). Microsatellite instability (MSI) might be a favorable predictor for treatment response, but comprehensive data on the prevalence of MSI in CCA are missing. The aim of the current study was to determine the prevalence of MSI in a German tertiary care hospital. Formalin-fixed paraffin-embedded tissue samples, obtained in the study period from 2007 to 2015 from patients with CCA undergoing surgical resection with curative intention at Johann Wolfgang Goethe University hospital, were examined. All samples were investigated immunohistochemically for the presence of MSI (expression of MLH1, PMS2, MSH2, and MSH6) as well as by pentaplex polymerase chain reaction for five quasimonomorphic mononucleotide repeats (BAT-25, BAT-26, NR-21, NR-22, and NR-24). In total, 102 patients were included, presenting intrahepatic (n = 35, 34.3%), perihilar (n = 42, 41.2%), and distal CCA (n = 25, 24.5%). In the immunohistochemical analysis, no loss of expression of DNA repair enzymes was observed. In the PCR-based analysis, one out of 102 patients was found to be MSI-high and one out of 102 was found to be MSI-low. Thus, MSI seems to appear rarely in CCA in Germany. This should be considered when planning immune-modulating therapy trials for patients with CCA.
KW  - microsatellite instability
KW  - cholangiocarcinoma
KW  - immunohistochemistry
KW  - PCR
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/46332
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-463324
SN  - 1422-0067
SN  - 1661-6596
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
VL  - 19
IS  - 5, Art. 1421
SP  - 1
EP  - 10
PB  - Molecular Diversity Preservation International
CY  - Basel
ER  -