TY  - JOUR
A1  - Laiño, Jonathan
A1  - Wangorsch, Andrea
A1  - Blanco Pérez, Frank Eliezer
A1  - Wolfheimer, Sonja
A1  - Krause, Maren
A1  - Flaczyk, Adam
A1  - Möller, Tobias-Maximilian
A1  - Tsai, Mindy
A1  - Galli, Stephen
A1  - Vieths, Stefan
A1  - Toda, Masako
A1  - Scheurer, Stephan
A1  - Schülke, Stefan
T1  - Targeting of immune cells by dual tlr2/7 ligands suppresses features of allergic th2 immune responses in mice
T2  - Journal of immunology research
N2  - Background. TLR ligands can promote Th1-biased immune responses, mimicking potent stimuli of viruses and bacteria. Aim. To investigate the adjuvant properties of dual TLR2/7 ligands compared to those of the mixture of both single ligands.
Methods. Dual TLR2/7 ligands: CL401, CL413, and CL531, including CL264 (TLR7-ligand) and Pam2CysK4 (TLR2-ligand), were used. Immune-modulatory capacity of the dual ligands with the individual ligands alone or as a mixture in mouse BMmDCs, BMmDC:TC cocultures, or BMCMCs was compared and assessed in naïve mice and in a mouse model of OVA-induced intestinal allergy.
Results. CL413 and CL531 induced BMmDC-derived IL-10 secretion, suppressed rOVA-induced IL-5 secretion from OVA-specific DO11.10 CD4+ TCs, and induced proinflammatory cytokine secretion in vivo. In contrast, CL401 induced considerably less IL-10 secretion and led to IL-17A production in BMmDC:TC cocultures, but not BMCMC IL-6 secretion, or IL-6 or TNF-α production in vivo. No immune-modulating effects were observed with single ligands. All dual TLR2/7 ligands suppressed DNP-induced IgE-and-Ag-specific mast cell degranulation. Compared to vaccination with OVA, vaccination with the mixture CL531 and OVA, significantly suppressed OVA-specific IgE production in the intestinal allergy model.
Conclusions. Based on beneficial immune-modulating properties, CL413 and CL531 may have utility as potential adjuvants for allergy treatment.
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/52555
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-525553
SN  - 2314-7156
SN  - 2314-8861
N1  - Copyright © 2017 Jonathan Laiño et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 2017
IS  - Art. 7983217
SP  - 1
EP  - 12
PB  - Hindawi
CY  - New York, NY
ER  -