TY  - JOUR
A1  - Wastl, Jürgen
A1  - Duin, Evert C.
A1  - Iuzzolino, Lucia
A1  - Dörner, Wolfgang
A1  - Link, Thomas
A1  - Hoffmann, Silke
A1  - Sticht, Heinrich
A1  - Dau, Holger
A1  - Lingelbach, Klaus
A1  - Maier, Uwe-G.
T1  - Eukaryotically encoded and chloroplast-located rubredoxin is associated with photosystem II
T2  - Journal of biological chemistry
N2  - We analyzed a eukaryotically encoded rubredoxin from the cryptomonad Guillardia theta and identified additional domains at the N- and C-termini in comparison to known prokaryotic paralogous molecules. The cryptophytic N-terminal extension was shown to be a transit peptide for intracellular targeting of the protein to the plastid, whereas a C-terminal domain represents a membrane anchor. Rubredoxin was identified in all tested phototrophic eukaryotes. Presumably facilitated by its C-terminal extension, nucleomorph-encoded rubredoxin (nmRub) is associated with the thylakoid membrane. Association with photosystem II (PSII) was demonstrated by co-localization of nmRub and PSII membrane particles and PSII core complexes and confirmed by comparative electron paramagnetic resonance measurements. The midpoint potential of nmRub was determined as +125 mV, which is the highest redox potential of all known rubredoxins. Therefore, nmRub provides a striking example of the ability of the protein environment to tune the redox potentials of metal sites, allowing for evolutionary adaption in specific electron transport systems, as for example that coupled to the PSII pathway.
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/75851
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-758510
SN  - 0021-9258
VL  - 275.2000
IS  - 39
SP  - 30058
EP  - 30063
PB  - American Society for Biochemistry and Molecular Biology Publications
CY  - Bethesda, Md
ER  -