TY  - JOUR
A1  - Klaus, Maja
A1  - Grininger, Martin
T1  - Engineering strategies for rational polyketide synthase design
T2  - Natural product reports
N2  - Covering: mid 1990s to 2018
Over the last two decades, diverse approaches have been explored to generate new polyketides by engineering polyketide synthases (PKSs). Although it has been proven possible to produce new compounds by designed PKSs, engineering strategies failed to make polyketides available via widely applicable rules and protocols. Still, organic synthetic routes have to be employed whenever new polyketides are needed for applications in medicine, agriculture, and industry. In light of the rising demand for commodity products from feedstock and for fast and cheap access to pharmaceutical compounds, the need for harnessing PKSs to produce such molecules is more urgent than ever before. In this review, we focus on a multitude of approaches to engineer modular PKSs by swapping and replacing PKS modules and domains, which we analyze in the light of recent structural and biochemical data. We conclude with an outlook on possible strategies on how to increase success rates of PKS engineering in future.
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63550
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-635505
SN  - 1460-4752
N1  - This work was supported by a Lichtenberg grant of the Volkswagen Foundation to M. G. (grant number 85701). Further support was received by the LOEWE program (Landes-Offensive zur Entwicklung wissenschaftlich-ökonomischer Exzellenz) of the state of Hesse conducted within the framework of the MegaSyn Research Cluster.
VL  - 35
IS  - 10
SP  - 1070
EP  - 1081
PB  - Soc.
CY  - London
ER  -