TY  - JOUR
A1  - Brandes, Ralf
A1  - Dueck, Anne
A1  - Engelhardt, Stefan
A1  - Kaulich, Manuel
A1  - Kupatt, Christian
A1  - De Angelis, Maria Teresa
A1  - Leisegang, Matthias
A1  - Le Noble, Ferdinand
A1  - Moretti, Alessandra
A1  - Müller, Oliver J.
A1  - Skryabin, Boris V.
A1  - Thum, Thomas
A1  - Wurst, Wolfgang
T1  - DGK and DZHK position paper on genome editing: basic science applications and future perspective
T2  - Basic research in cardiology
N2  - For a long time, gene editing had been a scientific concept, which was limited to a few applications. With recent developments, following the discovery of TALEN zinc-finger endonucleases and in particular the CRISPR/Cas system, gene editing has become a technique applicable in most laboratories. The current gain- and loss-of function models in basic science are revolutionary as they allow unbiased screens of unprecedented depth and complexity and rapid development of transgenic animals. Modifications of CRISPR/Cas have been developed to precisely interrogate epigenetic regulation or to visualize DNA complexes. Moreover, gene editing as a clinical treatment option is rapidly developing with first trials on the way. This article reviews the most recent progress in the field, covering expert opinions gathered during joint conferences on genome editing of the German Cardiac Society (DGK) and the German Center for Cardiovascular Research (DZHK). Particularly focusing on the translational aspect and the combination of cellular and animal applications, the authors aim to provide direction for the development of the field and the most frequent applications with their problems.
KW  - Animal models
KW  - CRISPR/Cas
KW  - Genome editing
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/74739
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-747393
SN  - 1435-1803
N1  - Open Access funding enabled and organized by Projekt DEAL. The initiative for this manuscript was supported by a grant from the DGK and the DZHK.
VL  - 116
IS  - 1, art. 2
SP  - 1
EP  - 20
PB  - Steinkopff ; Springer
CY  - [Darmstadt u.a.] ; Heidelberg
ER  -