TY  - JOUR
A1  - Gallus, Susanne
A1  - Lammers, Fritjof
A1  - Nilsson, Maria Anna
T1  - When genomics is not enough : experimental evidence for a decrease in LINE-1 activity during the evolution of Australian marsupials
T2  - Genome biology and evolution
N2  - he autonomous transposable element LINE-1 is a highly abundant element that makes up between 15% and 20% of therian mammal genomes. Since their origin before the divergence of marsupials and placental mammals, LINE-1 elements have contributed actively to the genome landscape. A previous in silico screen of the Tasmanian devil genome revealed a lack of functional coding LINE-1 sequences. In this study we present the results of an in vitro analysis from a partial LINE-1 reverse transcriptase coding sequence in five marsupial species. Our experimental screen supports the in silico findings of the genome-wide degradation of LINE-1 sequences in the Tasmanian devil, and identifies a high frequency of degraded LINE-1 sequences in other Australian marsupials. The comparison between the experimentally obtained LINE-1 sequences and reference genome assemblies suggests that conclusions from in silico analyses of retrotransposition activity can be influenced by incomplete genome assemblies from short reads.
KW  - LINE-1
KW  - marsupials
KW  - transposable element
KW  - retrotransposition
KW  - Tasmanian devil
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/44305
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-443058
SN  - 1759-6653
N1  - The Author 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
VL  - 8
IS  - 8
SP  - 2406
EP  - 2412
PB  - Oxford Univ. Press
CY  - Oxford
ER  -