TY  - INPR
A1  - Köppke, Julia
A1  - Keller, Luise-Elektra
A1  - Stuck, Michelle
A1  - Arnow, Nicolas Dominik
A1  - Bannert, Norbert
A1  - Cingöz, Oya
T1  - Direct translation of incoming retroviral RNA genomes
T2  - bioRxiv
N2  - Viruses that carry a positive-sense, single-stranded RNA translate their genomes after entering the host cell to produce viral proteins, with the exception of retroviruses. A distinguishing feature of retroviruses is reverse transcription, where the ssRNA genome serves as a template to synthesize a double-stranded DNA copy that subsequently integrates into the host genome. As retroviral RNAs are produced by the host transcriptional machinery and are largely indistinguishable from cellular mRNAs, we investigated the potential of incoming retroviral genomes to express proteins. Here we show through various biochemical methods that HIV-1 genomes are translated after entry, in case of minimal or full-length genomes, envelopes using different cellular entry pathways and in diverse cell types. Our findings challenge the dogma that retroviruses require reverse transcription to produce viral proteins. Synthesis of retroviral proteins in the absence of productive infection has significant implications for basic retrovirology, immune responses and gene therapy applications.
Y1  - 2023
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/83003
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-830038
UR  - https://www.biorxiv.org/content/10.1101/2023.05.02.539066v1
IS  - 2023.05.02.539066 Version 1
PB  - bioRxiv
ER  -