TY  - JOUR
A1  - Kechkar, Adel
A1  - Nair, Deepak
A1  - Heilemann, Mike
A1  - Choquet, Daniel
A1  - Sibarita, Jean-Baptiste
T1  - Real-Time analysis and visualization for single-molecule based super-resolution microscopy
T2  - PLoS One
N2  - Accurate multidimensional localization of isolated fluorescent emitters is a time consuming process in single-molecule based super-resolution microscopy. We demonstrate a functional method for real-time reconstruction with automatic feedback control, without compromising the localization accuracy. Compatible with high frame rates of EM-CCD cameras, it relies on a wavelet segmentation algorithm, together with a mix of CPU/GPU implementation. A combination with Gaussian fitting allows direct access to 3D localization. Automatic feedback control ensures optimal molecule density throughout the acquisition process. With this method, we significantly improve the efficiency and feasibility of localization-based super-resolution microscopy.
Y1  - 2013
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/29496
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-294966
SN  - 1932-6203
VL  - 8
IS  - (4): e62918
PB  - PLoS [u.a.]
CY  - Lawrence, Kan.
ER  -