TY  - JOUR
A1  - Nürenberg-Goloub, Elina
A1  - Heinemann, Holger
A1  - Gerovac, Milan
A1  - Tampé, Robert
T1  - Ribosome recycling is coordinated by processive events in two asymmetric ATP sites of ABCE1
T2  - Life science alliance
N2  - Ribosome recycling orchestrated by ABCE1 is a fundamental process in protein translation and mRNA surveillance, connecting termination with initiation. Beyond the plenitude of well-studied translational GTPases, ABCE1 is the only essential factor energized by ATP, delivering the energy for ribosome splitting via two nucleotide-binding sites by a yet unknown mechanism. Here, we define how allosterically coupled ATP binding and hydrolysis events in ABCE1 empower ribosome recycling. ATP occlusion in the low-turnover control site II promotes formation of the pre-splitting complex and facilitates ATP engagement in the high-turnover site I, which in turn drives the structural reorganization required for ribosome splitting. ATP hydrolysis and ensuing release of ABCE1 from the small subunit terminate the post-splitting complex. Thus, ABCE1 runs through an allosterically coupled cycle of closure and opening at both sites, consistent with a processive clamp model. This study delineates the inner mechanics of ABCE1 and reveals why various ABCE1 mutants lead to defects in cell homeostasis, growth, and differentiation.
KW  - ABC proteins
KW  - ATPases
KW  - conformational dynamics
KW  - mRNA translation
KW  - macromolecular complexes
KW  - molecular motors
KW  - ribosome quality control
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/47108
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-471082
SN  - 2575-1077
N1  - Copyright & Usage : © 2018 Nürenberg-Goloub et al. https://creativecommons.org/licenses/by/4.0/
This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
VL  - 1
IS  - 3, e201800095
SP  - 1
EP  - 12
PB  - Life Science Alliance, LLC
CY  - [Woodbury, NY]
ER  -