TY  - JOUR
A1  - Sanhaji, Mourad
A1  - Ritter, Andreas Hans
A1  - Belsham, Hannah R.
A1  - Friel, Claire T.
A1  - Roth, Susanne
A1  - Louwen, Frank
A1  - Yuan, Juping
T1  - Polo-like kinase 1 regulates the stability of the mitotic centromere-associated kinesin in mitosis
T2  - Oncotarget
N2  - Proper bi-orientation of chromosomes is critical for the accurate segregation of chromosomes in mitosis. A key regulator of this process is MCAK, the mitotic centromere-associated kinesin. During mitosis the activity and localization of MCAK are regulated by mitotic key kinases including Plk1 and Aurora B. We show here that S621 in the MCAK’s C-terminal domain is the major phosphorylation site for Plk1. This phosphorylation regulates MCAK’s stability and facilitates its recognition by the ubiquitin/proteasome dependent APC/CCdc20 pathway leading to its D-box dependent degradation in mitosis. While phosphorylation of S621 does not directly affect its microtubule depolymerising activity, loss of Plk1 phosphorylation on S621 indirectly enhances its depolymerization activity in vivo by stabilizing MCAK, leading to an increased level of protein. Interfering with phosphorylation at S621 causes spindle formation defects and chromosome misalignments. Therefore, this study suggests a new mechanism by which Plk1 regulates MCAK: by regulating its degradation and hence controlling its turnover in mitosis.
KW  - MCAK
KW  - Plk1
KW  - protein stability
KW  - chromosome alignment
KW  - spindle assembly
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/33562
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-335622
SN  - 1949-2553
N1  - Licensed under a Creative Commons Attribution 3.0 License. http://creativecommons.org/licenses/by/3.0/
VL  - 10
IS  - 5
SP  - 3130
EP  - 3144
PB  - Impact Journals LLC
CY  - [S.l.]
ER  -