TY  - JOUR
A1  - Posch, Wilfried
A1  - Steger, Marion
A1  - Knackmuss, Ulla
A1  - Blatzer, Michael
A1  - Baldauf, Hanna-Mari
A1  - Doppler, Wolfgang
A1  - White, Tommy E.
A1  - Hörtnagl, Paul
A1  - Diaz-Griffero, Felipe
A1  - Lass-Flörl, Cornelia
A1  - Hackl, Hubert
A1  - Moris, Arnaud
A1  - Keppler, Oliver Till
A1  - Wilflingseder, Doris
T1  - Complement-opsonized HIV-1 overcomes restriction in dendritic cells
T2  - PLoS pathogens
N2  - DCs express intrinsic cellular defense mechanisms to specifically inhibit HIV-1 replication. Thus, DCs are productively infected only at very low levels with HIV-1, and this non-permissiveness of DCs is suggested to go along with viral evasion. We now illustrate that complement-opsonized HIV-1 (HIV-C) efficiently bypasses SAMHD1 restriction and productively infects DCs including BDCA-1 DCs. Efficient DC infection by HIV-C was also observed using single-cycle HIV-C, and correlated with a remarkable elevated SAMHD1 T592 phosphorylation but not SAMHD1 degradation. If SAMHD1 phosphorylation was blocked using a CDK2-inhibitor HIV-C-induced DC infection was also significantly abrogated. Additionally, we found a higher maturation and co-stimulatory potential, aberrant type I interferon expression and signaling as well as a stronger induction of cellular immune responses in HIV-C-treated DCs. Collectively, our data highlight a novel protective mechanism mediated by complement opsonization of HIV to effectively promote DC immune functions, which might be in the future exploited to tackle HIV infection.
Y1  - 2015
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/38008
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-380087
SN  - 1553-7374
SN  - 1553-7366
N1  - Copyright: © 2015 Posch et al. This is an open access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 11
IS  - (6): e1005005
SP  - 1
EP  - 23
PB  - PLoS
CY  - Lawrence, Kan.
ER  -