TY  - JOUR
A1  - Sharma, Sunny
A1  - Yang, Jun
A1  - Düttmann, Simon
A1  - Watzinger, Peter
A1  - Kötter, Peter
A1  - Entian, Karl-Dieter
T1  - Identification of novel methyltransferases, Bmt5 and Bmt6, responsible for the m3U methylations of 25S rRNA in Saccharomyces cerevisiae
T2  - Nucleic acids research
N2  - RNA contains various chemical modifications that expand its otherwise limited repertoire to mediate complex processes like translation and gene regulation. 25S rRNA of the large subunit of ribosome contains eight base methylations. Except for the methylation of uridine residues, methyltransferases for all other known base methylations have been recently identified. Here we report the identification of BMT5 (YIL096C) and BMT6 (YLR063W), two previously uncharacterized genes, to be responsible for m3U2634 and m3U2843 methylation of the 25S rRNA, respectively. These genes were identified by RP-HPLC screening of all deletion mutants of putative RNA methyltransferases and were confirmed by gene complementation and phenotypic characterization. Both proteins belong to Rossmann-fold-like methyltransferases and the point mutations in the S-adenosyl-L-methionine binding pocket abolish the methylation reaction. Bmt5 localizes in the nucleolus, whereas Bmt6 is localized predominantly in the cytoplasm. Furthermore, we showed that 25S rRNA of yeast does not contain any m5U residues as previously predicted. With Bmt5 and Bmt6, all base methyltransferases of the 25S rRNA have been identified. This will facilitate the analyses of the significance of these modifications in ribosome function and cellular physiology.
Y1  - 2013
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/33823
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-338236
UR  - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3950682/
SN  - 1362-4962
SN  - 0305-1048
VL  - 42
IS  - 5
SP  - 3246
EP  - 3260
PB  - Oxford Univ. Press
CY  - Oxford
ER  -