TY  - JOUR
A1  - Michelsen, Kathrin S.
A1  - Aicher, Alexandra
A1  - Mohaupt, Mariette
A1  - Hartung, Thomas
A1  - Dimmeler, Stefanie
A1  - Kirschning, Carsten Jürgen
A1  - Schumann, Ralf R.
T1  - The role of toll-like receptors (TLRs) in bacteria-induced maturation of murine dendritic cells (DCS) : peptidoglycan and lipoteichoic acid are inducers of DC maturation and require TLR2
T2  - Journal of biological chemistry
N2  - Toll-like receptors (TLRs) have been found to be key elements in pathogen recognition by the host immune system. Dendritic cells (DCs) are crucial for both innate immune responses and initiation of acquired immunity. Here we focus on the potential involvement of TLR ligand interaction in DC maturation. TLR2 knockout mice and mice carrying a TLR4 mutation (C3H/HeJ) were investigated for DC maturation induced by peptidoglycan (PGN), lipopolysaccharide (LPS), or lipoteichoic acids (LTAs). All stimuli induced maturation of murine bone marrow-derived DCs in control mice. TLR2−/− mice lacked maturation upon stimulation with PGN, as assessed by expression of major histocompatibility complex class II, CD86, cytokine, and chemokine production, fluorescein isothiocyanate-dextran uptake, and mixed lymphocyte reactions, while being completely responsive to LPS. A similar lack of maturation was observed in C3H/HeJ mice upon stimulation with LPS. DC maturation induced by LTAs from two different types of bacteria was severely impaired in TLR2−/−, whereas C3H/HeJ mice responded to LTAs in a manner similar to wild-type mice. We demonstrate that DC maturation is induced by stimuli from Gram-positive microorganisms, such as PGN and LTA, with similar efficiency as by LPS. Finally, we provide evidence that TLR2 and TLR4 interaction with the appropriate ligand is essential for bacteria-induced maturation of DCs.
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/75937
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-759373
SN  - 0021-9258
VL  - 276
IS  - 28
SP  - 25680
EP  - 25686
PB  - American Society for Biochemistry and Molecular Biology Publications
CY  - Bethesda, Md
ER  -