TY  - JOUR
A1  - Brunnberg, Jamina
A1  - Herbring, Valentina
A1  - Günther Castillo, Esteban
A1  - Krüger, Heike
A1  - Wieneke, Ralph
A1  - Tampé, Robert
T1  - Light control of the peptide-loading complex synchronizes antigen translocation and MHC I trafficking
T2  - Communications biology
N2  - Antigen presentation via major histocompatibility complex class I (MHC I) molecules is essential to mount an adaptive immune response against pathogens and cancerous cells. To this end, the transporter associated with antigen processing (TAP) delivers snippets of the cellular proteome, resulting from proteasomal degradation, into the ER lumen. After peptide loading and editing by the peptide-loading complex (PLC), stable peptide-MHC I complexes are released for cell surface presentation. Since the process of MHC I trafficking is poorly defined, we established an approach to control antigen presentation by introduction of a photo-caged amino acid in the catalytic ATP-binding site of TAP. By optical control, we initiate TAP-dependent antigen translocation, thus providing new insights into TAP function within the PLC and MHC I trafficking in living cells. Moreover, this versatile approach has the potential to be applied in the study of other cellular pathways controlled by P-loop ATP/GTPases.
KW  - Chemical modification
KW  - Membranes
KW  - Transporters
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/75124
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-751243
SN  - 2399-3642
N1  - The work was supported by the German Research Foundation (GRK 1986 to R.W. and R.T.; TA 157/12-1 and CRC 807—Membrane Transport and Communication to R.T.), as well as by the European Research Council (ERC Advanced Grant 789121 to R.T.). Open Access funding enabled and organized by Projekt DEAL.
VL  - 4
IS  - art. 430
SP  - 1
EP  - 10
PB  - Springer Nature
CY  - London
ER  -